Revistas
Revista:
STEM CELL RESEARCH
ISSN:
1873-5061
Año:
2023
Vol.:
71
Págs.:
103189
Transthyretin (TTR) amyloid cardiomyopathy (ATTR-CM) is a life-threatening disease caused by the abnormal production of misfolded TTR protein by liver cells, which is then released systemically. Its amyloid deposition in the heart is linked to cardiac toxicity and progression toward heart failure. A human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) from a patient suffering familial transthyretin amyloid cardio-myopathy carrying a c.128G>A (p.Ser43Asn) mutation in the TTR gene. This iPSC line offers a useful resource to study the disease pathophysiology and a cell-based model for therapeutic discovery.
Revista:
BIOFABRICATION
ISSN:
1758-5082
Año:
2022
Vol.:
14
N°:
4
Págs.:
045017
Biofabrication of human tissues has seen a meteoric growth triggered by recent technical advancements such as human induced pluripotent stem cells (hiPSCs) and additive manufacturing. However, generation of cardiac tissue is still hampered by lack of adequate mechanical properties and crucially by the often unpredictable post-fabrication evolution of biological components. In this study we employ melt electrowriting (MEW) and hiPSC-derived cardiac cells to generate fibre-reinforced human cardiac minitissues. These are thoroughly characterized in order to build computational models and simulations able to predict their post-fabrication evolution. Our results show that MEW-based human minitissues display advanced maturation 28 post-generation, with a significant increase in the expression of cardiac genes such as MYL2, GJA5, SCN5A and the MYH7/MYH6 and MYL2/MYL7 ratios. Human iPSC-cardiomyocytes are significantly more aligned within the MEW-based 3D tissues, as compared to conventional 2D controls, and also display greater expression of C x43. These are also correlated with a more mature functionality in the form of faster conduction velocity. We used these data to develop simulations capable of accurately reproducing the experimental performance. In-depth gauging of the structural disposition (cellular alignment) and intercellular connectivity (C x43) allowed us to develop an improved computational model able to predict the relationship between cardiac cell alignment and functional performance. This study lays down the path for advancing in the development of in silico tools to predict cardiac biofabricated tissue evolution after generation, and maps the route towards more accurate and biomimetic tissue manufacture.
Revista:
ACS OMEGA
ISSN:
2470-1343
Año:
2019
Vol.:
4
N°:
6
Págs.:
10593 - 10598
An efficient multienzyme system for the preparatiy e synthesis of D-xylonate, a chemical with versatile industrial applications, is described. The multienzyme system is based on D-xylose oxidation catalyzed by the xylose dehydrogenase from Calulobacter crescentus and the use of catalytic amounts of NAD(+). The cofactor is regenerated in situ by coupling the reduction of acetaldehyde into ethanol catalyzed by alcohol dehydrogenase from Clostridium kluyveri. Excellent conversions (>95%) were obtained in a process that allows easy product isolation by simple evaporation of the volatile buffer and byproducts.
Nacionales y Regionales
Título:
Bioingeniería humana para la detección y análisis de toxicidad cardiovascular secundaria a terapias anti-oncológias. Análisis del factor sexo.
Código de expediente:
GN32/2023
Investigador principal:
Manuel María Mazo Vega
Financiador:
GOBIERNO DE NAVARRA. DEPARTAMENTO DE SALUD
Convocatoria:
2023 GN Proyectos de Investigación en salud
Fecha de inicio:
19/12/2023
Fecha fin:
18/12/2026
Importe concedido:
79.925,00€
Otros fondos:
-
Título:
Bioingenieria personalizada para el tratamiento de las enfermedades cardiovasculares. Estudio de la implicación del género
Código de expediente:
0011-1383-2022-000015(PC020-21-022 BIOGEN)
Investigador principal:
Manuel María Mazo Vega
Financiador:
GOBIERNO DE NAVARRA
Convocatoria:
2022 GN Proyectos Colaborativos
Fecha de inicio:
01/12/2021
Fecha fin:
30/11/2024
Importe concedido:
211.870,50€
Otros fondos:
-
Título:
Tecnología 3D en bioingeniería de tejidos para generación de un miocardio humano maduro
Código de expediente:
0011-1383-2018-000011
Investigador principal:
Manuel María Mazo Vega
Financiador:
GOBIERNO DE NAVARRA
Convocatoria:
2018 - GN INDUSTRIA PROYECTOS CENTROS TECNOLOGICOS Y ORGANISMOS DE INVESTIGACION
Fecha de inicio:
01/02/2018
Fecha fin:
30/11/2018
Importe concedido:
149.604,75€
Otros fondos:
-